6GB7
Structure of H-2Db with scoop loop from tapasin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-07 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.700, 123.860, 100.110 |
Unit cell angles | 90.00, 103.66, 90.00 |
Refinement procedure
Resolution | 52.242 - 2.150 |
R-factor | 0.2404 |
Rwork | 0.239 |
R-free | 0.26590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s7u |
RMSD bond length | 0.004 |
RMSD bond angle | 0.658 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.280 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.113 | 0.506 |
Number of reflections | 117221 | 5702 |
<I/σ(I)> | 5.8 | 2 |
Completeness [%] | 98.4 | 97.9 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1.8M ammonium sulfate, 0.1M Tris-HCl (pH 8) and 0.5M NaCl |