6G32
Crystal structure of human geranylgeranyl diphosphate synthase mutant D188Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-08 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 148.540, 148.540, 268.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.901 - 3.281 |
| R-factor | 0.2085 |
| Rwork | 0.206 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q80 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.893 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.901 | 3.206 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.205 | |
| Number of reflections | 50043 | |
| <I/σ(I)> | 7.76 | |
| Completeness [%] | 98.4 | |
| Redundancy | 4.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 3.6M Sodium formate, 0.01% w/v aspartame, 0.01% w/v gly-gly-gly, 0.01% w/v pentaglycine, 0.01% w/v tyr-ala, 0.01% w/v tyr-phe, 0.0018M HEPES sodium pH 6.8 |
