6FMF
Neuropilin-1 b1 domain in complex with EG01377; 2.8 Angstrom structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-01 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 41 |
| Unit cell lengths | 39.655, 39.655, 91.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.671 - 2.811 |
| R-factor | 0.2351 |
| Rwork | 0.233 |
| R-free | 0.29230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kex |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.335 |
| Data reduction software | d*TREK |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.650 | 2.960 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.180 | 0.350 |
| Rpim | 0.075 | |
| Number of reflections | 3271 | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 98.1 | 82.2 |
| Redundancy | 12.8 | |
| CC(1/2) | 0.994 | 0.938 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 16 % w/v PEG3350 and 200 mM ammonium chloride |
