6F09
Binary complex of 14-3-3 zeta with ubiquitin specific protease 8 (USP8) pSer718 peptide
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.379, 64.890, 71.519 |
| Unit cell angles | 99.71, 114.22, 108.79 |
Refinement procedure
| Resolution | 61.137 - 1.594 |
| R-factor | 0.1822 |
| Rwork | 0.180 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hkc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.011 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.137 | 1.620 |
| High resolution limit [Å] | 1.594 | 1.594 |
| Rmerge | 0.062 | |
| Rmeas | 0.088 | |
| Rpim | 0.062 | |
| Number of reflections | 107299 | |
| <I/σ(I)> | 1.84 | |
| Completeness [%] | 91.5 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.1 M phosphate citrate pH 4.4; 40% PEG 300 |
