6F00

Crystal Structure of human tRNA dihydrouridine synthase (20) dsRBD E423A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.976254
Spacegroup name	P 1
Unit cell lengths	52.250, 52.210, 52.310
Unit cell angles	107.69, 107.92, 107.65

Refinement procedure

Resolution	44.700 - 2.163
R-factor	0.1791
Rwork	0.176
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wft
RMSD bond length	0.013
RMSD bond angle	1.102
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	2.240
High resolution limit [Å]	2.163	2.163
Rmerge	0.072	0.641
Number of reflections	41560	2174
<I/σ(I)>	7.58	1.3
Completeness [%]	96.0	92.16
Redundancy	2	2
CC(1/2)	0.995	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	1.6 M ammonium sulfate 100 mM sodium chloride 100 mM HEPES