6EZC
Crystal Structure of human tRNA-dihydrouridine(20) synthase catalytic domain E294K Q305K double mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-03 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.980066 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.990, 84.660, 100.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.330 - 2.000 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xp7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS (20151015) |
Data scaling software | STARANISO (1.10.15) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.170 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.161 | 2.521 |
Rmeas | 0.170 | 2.665 |
Number of reflections | 22677 | 1135 |
<I/σ(I)> | 10.1 | 1.1 |
Completeness [%] | 89.9 | 43.5 |
Redundancy | 9.3 | 8.9 |
CC(1/2) | 0.997 | 0.413 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 30% PEG 2000 MME 200 mM ammonium sulfate 50 mM sodium acetate |