6EXW
Crystal structure of cIAP1-BIR3 in complex with a covalently bound SM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.799, 53.799, 176.627 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.880 - 2.200 |
| R-factor | 0.1939 |
| Rwork | 0.191 |
| R-free | 0.25140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mup |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.651 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.900 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.077 | 0.551 |
| Rmeas | 0.148 | 0.630 |
| Number of reflections | 15655 | 2254 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.9 | 293 | 12% PEG 3350, 0.22 M MgCl2, 0.1M BISTris |
