6EOR
DPP9 - 1G244
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99997 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 119.357, 117.212, 163.396 |
| Unit cell angles | 90.00, 105.57, 90.00 |
Refinement procedure
| Resolution | 43.520 - 2.900 |
| R-factor | 0.26852 |
| Rwork | 0.265 |
| R-free | 0.33173 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1orv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.335 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 3.090 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 95096 | 16260 |
| <I/σ(I)> | 9.5 | 4.98 |
| Completeness [%] | 99.0 | 99.2 |
| Redundancy | 4.28 | 4.38 |
| CC(1/2) | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% PEG 8000, 25% Glycerol, 0.16M Calcium Acetate, 0.08M Cacodilate pH 6.25 |
