6EKK
Crystal structure of GEF domain of DENND 1A in complex with Rab GTPase Rab35-GDP bound state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.700, 67.990, 101.710 |
| Unit cell angles | 80.50, 76.80, 67.06 |
Refinement procedure
| Resolution | 50.452 - 1.820 |
| R-factor | 0.1954 |
| Rwork | 0.194 |
| R-free | 0.23230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tw8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.104 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.452 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 115286 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 95.7 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25%PEG3350, 0.2M AMS, 0.1M Bis-Tris pH 5.5 |
