6EJ0
Crystal structure of KDM5B in complex with KDOPZ000049a.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 141.820, 141.820, 151.750 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.807 - 2.060 |
| R-factor | 0.1914 |
| Rwork | 0.190 |
| R-free | 0.21040 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.979 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.240 | 2.110 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.094 | 2.416 |
| Number of reflections | 56074 | 4074 |
| <I/σ(I)> | 21.6 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.8 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES pH 7.5, 0.8M Pottassium Phosphate-dibasic, 0.8M Sodium Phosphate monobasic |
