6EIY
Crystal structure of KDM5B in complex with KDOPZ000034a.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 142.380, 142.380, 152.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.983 - 2.150 |
R-factor | 0.1876 |
Rwork | 0.186 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a1f |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.983 | 2.210 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.116 | 2.524 |
Number of reflections | 49958 | 3615 |
<I/σ(I)> | 18.7 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.6 | 19 |
CC(1/2) | 0.586 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES pH 7.5, 0.8M Pottassium Phosphate-dibasic, 0.8M Sodium Phosphate monobasic |