6EIM
Human STK10 bound to GW683134A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.468, 99.812, 125.839 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.806 - 1.430 |
| R-factor | 0.1554 |
| Rwork | 0.154 |
| R-free | 0.17360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j7t |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.676 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.29) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.390 | 46.390 | 1.480 |
| High resolution limit [Å] | 1.430 | 5.540 | 1.430 |
| Rmerge | 0.053 | 0.034 | 0.839 |
| Rmeas | 0.061 | 0.039 | 0.958 |
| Rpim | 0.028 | 0.018 | 0.450 |
| Total number of observations | 449512 | ||
| Number of reflections | 110558 | 1886 | 10616 |
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 96.0 | 87.9 | 94.8 |
| Redundancy | 4.1 | 4 | 3.9 |
| CC(1/2) | 0.999 | 0.998 | 0.535 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10% PEG1000, 10% PEG8000 |
