6EIE

Ras guanine nucleotide exchange factor SOS2 (Rem-cdc25), with surface mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0332
Spacegroup name	P 43
Unit cell lengths	53.970, 53.970, 183.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.540 - 2.720
R-factor	0.22171
Rwork	0.218
R-free	0.28526
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nvu
RMSD bond length	0.008
RMSD bond angle	1.244
Data reduction software	XDS (VERSION Oct 15, 2015)
Data scaling software	XDS (VERSION Oct 15, 2015)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.540	2.880
High resolution limit [Å]	2.720	2.720
Number of reflections	13644	1867
<I/σ(I)>	14.1
Completeness [%]	96.9	82.7
Redundancy	4.8	3.2
CC(1/2)	0.998	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Protein 10.3 mg/ml in 25 mM Tris HCl, pH 7.5, 100 mM NaCl, 1 mM DTT. Reservoir 0,1 M Natriumacetat, 20% w/v PEG 3350. Cryo buffer was reservoir supplemented with 15% glycerol.