6EHT
Modulation of PCNA sliding surface by p15PAF suggests a suppressive mechanism for cisplatin-induced DNA lesion bypass by pol eta holoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.993, 42.300, 141.827 |
| Unit cell angles | 90.00, 102.70, 90.00 |
Refinement procedure
| Resolution | 40.480 - 3.200 |
| R-factor | 0.26401 |
| Rwork | 0.261 |
| R-free | 0.32715 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d2g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.396 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.120 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.105 | 0.320 |
| Number of reflections | 14271 | 2122 |
| <I/σ(I)> | 5.7 | 2.2 |
| Completeness [%] | 95.3 | 98.8 |
| Redundancy | 2.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% polyethylene glycol 3350 0.1 M sodium acetate pH 4.5 |
