6EHG
complement component C3b in complex with a nanobody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.98021 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 255.310, 64.880, 144.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.880 - 2.650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fo7 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 62.880 |
High resolution limit [Å] | 2.650 |
Number of reflections | 70861 |
<I/σ(I)> | 17.42 |
Completeness [%] | 99.8 |
Redundancy | 40.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 12 mM Sodium Citrate pH 8, 28 mM Sodium Citrate pH 6.0, 12.25 % (w/v) PEG 2000 MME, 70 mM Imidazole pH 7, 60 mM Ammonium acetate, 70 mM Tris pH 8.5, 13.5 % (v/v) and (+/-)-2-Methyl-2,4-pentanediol |