6EGS

Crystal structure of the GalNAc-T2 F104S mutant in complex with UDP-GalNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	154.036, 154.036, 109.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	154.040 - 2.700
R-factor	0.20669
Rwork	0.206
R-free	0.22104
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d0t
RMSD bond length	0.011
RMSD bond angle	1.529
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	154.040	2.850
High resolution limit [Å]	2.700	2.700
Rpim	0.064	0.720
Number of reflections	70380
<I/σ(I)>	7.8	1.1
Completeness [%]	99.7	100
Redundancy	5.6	5.5
CC(1/2)	0.995	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG6000, Hepes