6EG8

Structure of the GDP-bound Gs heterotrimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-08-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	100.860, 100.810, 179.330
Unit cell angles	90.00, 106.18, 90.00

Refinement procedure

Resolution	48.433 - 2.800
R-factor	0.2182
Rwork	0.217
R-free	0.26940
RMSD bond length	0.002
RMSD bond angle	0.493
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3228)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.440	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	85278
<I/σ(I)>	4.28
Completeness [%]	99.9
Redundancy	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	17.5%(w/v) PEG 3350, 0.1M MgCl2