6EG0

Crystal structure of Dpr4 Ig1-Ig2 in complex with DIP-Eta Ig1-Ig3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-10
Detector	ADSC QUANTUM 315
Wavelength(s)	.97918
Spacegroup name	P 31 2 1
Unit cell lengths	94.294, 94.294, 212.463
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.987 - 2.900
R-factor	0.2233
Rwork	0.221
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5EO9 4cmm & 5K6U
RMSD bond length	0.002
RMSD bond angle	0.492
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.200	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.114	0.686
Number of reflections	24914	3936
<I/σ(I)>	9.7
Completeness [%]	99.5
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	28% Edo_p8k (Molecular Dimensions), 0.1 M Morpheus Buffer 2, pH 7.5 (Molecular Dimensions), 10% carboxylic acid (Molecular Dimensions)