6EDW
Crystal structure of Mycobacterium tuberculosis ICL2 in the apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.824, 136.078, 138.924 |
| Unit cell angles | 90.00, 99.59, 90.00 |
Refinement procedure
| Resolution | 48.320 - 1.800 |
| R-factor | 0.16999 |
| Rwork | 0.168 |
| R-free | 0.20254 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.988 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 136.980 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 323074 | |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 9% w/v PEG 4K, 18% v/v glycerol, 0.02 M amino acids, 0.1 M MES/imidazole pH 6.9 |
