6EDB

Crystal structure of SRY.hcGAS-21bp dsDNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	I 2 2 2
Unit cell lengths	90.307, 115.827, 159.077
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.008 - 3.209
R-factor	0.2598
Rwork	0.255
R-free	0.30160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k96
RMSD bond length	0.019
RMSD bond angle	0.568
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.008	3.320
High resolution limit [Å]	3.200	3.200
Rpim	0.051	0.338
Number of reflections	12639	833
<I/σ(I)>	13.4
Completeness [%]	60.4
Redundancy	5.4
CC(1/2)	0.980	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M L-proline, 0.1 M HEPES, pH 7.5, 8% PEG3350 (v/v), 10 mM MgCl2