6EA7

Structure of EBOV GPcl in complex with the pan-ebolavirus mAb ADI-15878

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 31 2 1
Unit cell lengths	152.781, 152.781, 247.685
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.080 - 4.250
R-factor	0.2832
Rwork	0.280
R-free	0.32390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hj3
RMSD bond length	0.003
RMSD bond angle	0.655
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.080	4.400
High resolution limit [Å]	4.250	4.250
Number of reflections	24218	4317
<I/σ(I)>	10.6	2.1
Completeness [%]	99.9	100
Redundancy	173.1	162.8
CC(1/2)	1.000	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	100 mM MgCl2, 100 mM HEPES pH 7.5, and 10% PEG 4000