6E7I

Human ppGalNAcT2 I253A/L310A Mutant with EA2 and UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	95
Detector technology	PIXEL
Collection date	2017-08-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97741
Spacegroup name	P 61
Unit cell lengths	69.310, 69.310, 169.780
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.660 - 1.800
R-factor	0.1696
Rwork	0.167
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ffu
RMSD bond length	0.013
RMSD bond angle	1.605
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.020	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.107	0.471
Number of reflections	42632	5819
<I/σ(I)>	5.3	0.715
Completeness [%]	92.1	93.7
Redundancy	2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG8000, HEPES