6E2P

Structure of human JAK2 FERM/SH2 in complex with Leptin Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 65 2 2
Unit cell lengths	263.870, 263.870, 101.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.186 - 2.830
R-factor	0.228
Rwork	0.227
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z32
RMSD bond length	0.003
RMSD bond angle	0.603
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1-2155_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.190	2.931
High resolution limit [Å]	2.830	2.830
Rmerge	0.105	1.597
Rmeas	0.109	1.658
Rpim	0.029	0.441
Number of reflections	49544	4861
<I/σ(I)>	21.83	2.03
Completeness [%]	99.6	99.47
Redundancy	13.4	13.7
CC(1/2)	0.999	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	0.1 M MES, pH 6.5, 0.2 M magnesium chloride, 5-10% PEG4000, 10% ethylene glycol