6DS6

Crystal structure of p300 ZZ domain in complex with histone H3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-04-22
Detector	RDI CMOS_8M
Wavelength(s)	1.278
Spacegroup name	P 43 21 2
Unit cell lengths	44.026, 44.026, 85.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.320 - 1.950
R-factor	0.2117
Rwork	0.207
R-free	0.25320
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.922
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.180	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.077	0.296
Number of reflections	6576	292
<I/σ(I)>	71.4
Completeness [%]	99.2
Redundancy	22.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M HEPES and 70% MPD (pH 7.5)