6DOV

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 80 s at 21 C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8
Spacegroup name	C 1 2 1
Unit cell lengths	81.656, 37.396, 61.670
Unit cell angles	90.00, 96.51, 90.00

Refinement procedure

Resolution	20.058 - 1.521
R-factor	0.1547
Rwork	0.153
R-free	0.18960
Starting model (for MR)	pdbid 1ZBL
RMSD bond length	0.008
RMSD bond angle	1.155
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.060	20.060	1.550
High resolution limit [Å]	1.520	8.330	1.520
Rmerge	0.033	0.019	0.744
Rmeas	0.047	0.027	1.052
Rpim	0.033	0.019	0.744
Total number of observations	53577
Number of reflections	28106	189	1005
<I/σ(I)>	10.3
Completeness [%]	97.9	93.2	69.5
Redundancy	1.9	1.8	1.7
CC(1/2)	0.999	0.998	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2