6DO3
KLHDC2 ubiquitin ligase in complex with SelK C-end degron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.816, 87.779, 88.626 |
Unit cell angles | 90.00, 104.49, 90.00 |
Refinement procedure
Resolution | 43.391 - 2.165 |
R-factor | 0.1691 |
Rwork | 0.166 |
R-free | 0.22320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vpj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.160 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.190 |
High resolution limit [Å] | 2.150 | 5.830 | 2.150 |
Rmerge | 0.168 | 0.084 | 0.598 |
Rmeas | 0.182 | 0.091 | 0.679 |
Rpim | 0.069 | 0.036 | 0.316 |
Number of reflections | 35125 | 1817 | 1759 |
<I/σ(I)> | 6.4 | ||
Completeness [%] | 99.9 | 99.8 | 99.2 |
Redundancy | 6.9 | 6.7 | 4.3 |
CC(1/2) | 0.995 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.03M MgCl2*6H2O, 0.03M CaCl2*2H2O, 0.05M Imidazole, 0.05M MES monohydrate, 12.5% v/v MPD, 12.5% PEG1000, 12.5% w/v PEG3350, pH 6.5. |