6DLD

Crystal structure of IgLON5/NEGR1 heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	120.341, 305.799, 60.029
Unit cell angles	90.00, 100.78, 90.00

Refinement procedure

Resolution	48.596 - 3.300
R-factor	0.247
Rwork	0.245
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eo9
RMSD bond length	0.003
RMSD bond angle	0.569
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	3.500
High resolution limit [Å]	3.300	3.300
Number of reflections	31695
<I/σ(I)>	7.92
Completeness [%]	98.8
Redundancy	3.5
CC(1/2)	0.996	0.397

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	MES-NaOH pH6.5, MgSO4