6DFY

Remodeled crystal structure of DNA-bound DUX4-HD2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-15
Detector	MAR CCD 130 mm
Wavelength(s)	0.9747
Spacegroup name	P 41 21 2
Unit cell lengths	51.610, 51.610, 166.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.297 - 2.623
R-factor	0.2475
Rwork	0.246
R-free	0.27940
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5z2t
RMSD bond length	0.006
RMSD bond angle	0.973
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((dev_3139: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.300	2.760
High resolution limit [Å]	2.620	2.620
Rmerge		1.500
Number of reflections	7363	1026
<I/σ(I)>	11.5
Completeness [%]	99.9	100
Redundancy	9.5	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG8000