6DC9
Fab/epitope complex of human chimeric monoclonal antibody h4E6 targeting a phosphorylated tau epitope.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-18 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.212, 65.080, 97.439 |
Unit cell angles | 90.00, 112.11, 90.00 |
Refinement procedure
Resolution | 48.692 - 2.999 |
R-factor | 0.208 |
Rwork | 0.202 |
R-free | 0.25990 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.595 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.690 | 3.110 |
High resolution limit [Å] | 2.990 | 2.990 |
Number of reflections | 18575 | |
<I/σ(I)> | 10.15 | |
Completeness [%] | 99.4 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 25.5% polyethylene glycol 4000 and 0.17 M ammonium sulfate |