6DC4

RSV-neutralizing human antibody AM22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-25
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	64.240, 75.220, 109.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.720 - 1.700
R-factor	0.1812
Rwork	0.180
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lmj 3qeg
RMSD bond length	0.006
RMSD bond angle	0.836
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER (2.8.0)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.720	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	58786	20172
<I/σ(I)>	17.4	2.3
Completeness [%]	100.0	100
Redundancy	7.1	6.5
CC(1/2)	0.999	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.02 M 1,6-Hexanediol, 0.02 M 1-Butanol, 0.02 M 1,2-Propanediol (racemic), 0.02 M 2-Propanol, 0.02 M 1,4-Butanediol, 0.02 M 1,3-Propanediol, 0.1 M MES/imidazole pH 6.5, 10% PEG 8000, 20% ethylene glycol