6D7M
Crystal structure of the W184R/W231R Importin alpha mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-07 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.514 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.813, 90.162, 98.394 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.907 - 2.187 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e6q |
RMSD bond length | 0.006 |
RMSD bond angle | 0.808 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.187 | 2.200 |
Rmeas | 0.843 | |
Rpim | 0.043 | 0.336 |
Number of reflections | 35979 | 1705 |
<I/σ(I)> | 9.2 | 2.2 |
Completeness [%] | 99.1 | 96.5 |
Redundancy | 6.3 | 6 |
CC(1/2) | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Bis-Tris-Propane, 1.0M Sodium Succinate |