6D0Y

X-ray Crystal Structure of PGC-1beta C-terminus bound to the CBP80-CBP20 Cap Binding Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9553
Spacegroup name	P 21 21 21
Unit cell lengths	78.106, 111.897, 124.795
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.053 - 2.676
R-factor	0.185
Rwork	0.181
R-free	0.24010
RMSD bond length	0.004
RMSD bond angle	0.552
Data reduction software	XDS
Data scaling software	Aimless (0.5.4)
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.050	39.050	2.800
High resolution limit [Å]	2.670	8.870	2.670
Rmerge	0.211	0.067	1.188
Rmeas	0.234	0.074	1.338
Rpim	0.100	0.032	0.602
Total number of observations	167278	4903	17492
Number of reflections	30950	961	3554
<I/σ(I)>	5.9	10.5	2.1
Completeness [%]	97.9	98.5	86.2
Redundancy	5.4	5.1	4.9
CC(1/2)	0.980	0.991	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M magnesium chloride, 0.1 M Tris (pH 7.0), 10% [w/v] polyethylene glycol 8000