6CXZ

RNA octamer containing 2'-F, 4'-Calpha-Me U.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9184
Spacegroup name	P 43 21 2
Unit cell lengths	30.460, 30.460, 81.889
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.439 - 1.500
R-factor	0.1877
Rwork	0.186
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vr4
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2992)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.101	0.052	0.988
Rmeas	0.104	0.053	1.040
Rpim	0.022	0.012	0.313
Total number of observations	145275
Number of reflections	6684	417	328
<I/σ(I)>	8.7
Completeness [%]	99.8	100	99.7
Redundancy	21.6	21.1	9.5
CC(1/2)		0.999	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.0 mM RNA, 40 mM Na cacodylate, 20 mM magnesium chloride, 20 mM cobalt(3+) hexamine chloride, 40% MPD