6CUU

Thermus thermophiles RNA polymerase in complex with promoter DNA and antibiotic Kanglemycin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	185.221, 101.315, 294.279
Unit cell angles	90.00, 98.89, 90.00

Refinement procedure

Resolution	46.305 - 2.994
R-factor	0.202
Rwork	0.201
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.089
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.304	2.864
High resolution limit [Å]	2.794	2.794
Number of reflections	127584
<I/σ(I)>	7.78
Completeness [%]	95.5
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M Tris-HCl (pH 8.0), 0.2 M KCl, 50 mM MgCl2, 9.5% PEG4000