6CRF
Crystal Structure of Shp2 E76K GOF Mutant in the Open Conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 249.104, 41.735, 153.853 |
| Unit cell angles | 90.00, 125.76, 90.00 |
Refinement procedure
| Resolution | 124.840 - 2.620 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2shp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 124.840 | 2.630 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Number of reflections | 39327 | |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M MES pH 6.5, 12% PEG 20,000, and 5% Sucrose |
