6CPM

Structure of the USP15 deubiquitinase domain in complex with a third-generation inhibitory Ubv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-06-22
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	42.552, 115.080, 95.701
Unit cell angles	90.00, 92.59, 90.00

Refinement procedure

Resolution	38.205 - 2.011
R-factor	0.1681
Rwork	0.167
R-free	0.21170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	usp15 and Ubv model for the USP15/AM5 structure
RMSD bond length	0.007
RMSD bond angle	0.874
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.080
High resolution limit [Å]	2.010	2.010
Rmerge	0.067	0.507
Number of reflections	60994	6036
<I/σ(I)>	23.4	2.96
Completeness [%]	99.9	99.5
Redundancy	3.7	3.5
CC(1/2)		0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	16% PEG3350, 100 mM MES, 200 mM CaCl2, cryoprotected with the identical buffer plus 25% glycerol