6CLV
Staphylococcus aureus Dihydropteroate Synthase (saDHPS) F17L E208K double mutant structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979180 |
Spacegroup name | P 43 |
Unit cell lengths | 79.750, 79.750, 175.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.432 - 2.300 |
R-factor | 0.2119 |
Rwork | 0.191 |
R-free | 0.20990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.604 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.432 | 2.344 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 201103 | |
<I/σ(I)> | 10.8 | |
Completeness [%] | 99.7 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M Sodium Nitrate, and 20% PEG 3350 |