6C5Z
Human UDP-Glucose Dehydrogenase A225L substitutuion with UDP-glucose and NADH bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-04-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 93.770, 199.800, 200.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.061 - 2.950 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q3e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.007 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.063 | 3.020 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmeas | 0.167 | 1.142 |
| Number of reflections | 79604 | 5781 |
| <I/σ(I)> | 7.91 | 1.44 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 4.63 | 4.2 |
| CC(1/2) | 0.992 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 20% PEG3350, 50 mM dipotassium phosphate, 100 mM sodium glycine |
