6C5L

Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	211.023, 452.527, 623.443
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.981 - 3.200
R-factor	0.2241
Rwork	0.223
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v5j
RMSD bond length	0.004
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.250
High resolution limit [Å]	3.200	3.200
Rmeas	0.439	3.111
Number of reflections	970373	43805
<I/σ(I)>	10.29	1.04
Completeness [%]	100.0	100
Redundancy	41.9	29.8
CC(1/2)	0.999	0.479

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME