6C16
Ubiquitin variant (UbV.Fbl10.1) bound to a human Skp1-Fbl11 fragment complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 93.15 |
| Detector technology | PIXEL |
| Collection date | 2017-03-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.241, 119.582, 63.707 |
| Unit cell angles | 90.00, 98.45, 90.00 |
Refinement procedure
| Resolution | 19.818 - 3.270 |
| R-factor | 0.2632 |
| Rwork | 0.260 |
| R-free | 0.31720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.615 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.820 | 3.530 |
| High resolution limit [Å] | 3.270 | 3.270 |
| Number of reflections | 8718 | 1811 |
| <I/σ(I)> | 5.2 | 1.1 |
| Completeness [%] | 98.9 | 99.3 |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 0.986 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1 M malic acid pH 4.5, 0.15 M sodium chloride, 27% (w/v) PEG3350 |
