6BYR
Structures of the PKA RI alpha holoenzyme with the FLHCC driver J-PKAc alpha or native PKAc alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-10 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 166.504, 166.504, 332.701 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.914 - 3.661 |
| R-factor | 0.2028 |
| Rwork | 0.200 |
| R-free | 0.24950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2QCS and 4WB7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.790 |
| High resolution limit [Å] | 3.660 | 3.660 |
| Number of reflections | 30877 | 3018 |
| <I/σ(I)> | 29.5 | 8.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.3 | 22.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM NaCl, 16-18% pentaerythritol propoxylate and 10% dimethyl sulfoxide |
