6BYH
Ubiquitin Variant (UbV.Fl11.1) bound to a human Skp1-Fbl11 fragment complex.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E DW |
Temperature [K] | 93.15 |
Detector technology | CCD |
Collection date | 2016-09-30 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.560, 118.188, 146.336 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.090 - 2.610 |
R-factor | 0.238 |
Rwork | 0.235 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq 5jh5 5ibk |
RMSD bond length | 0.007 |
RMSD bond angle | 0.859 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.610 | 2.610 |
Number of reflections | 32211 | |
<I/σ(I)> | 24.7 | 1.7 |
Completeness [%] | 98.5 | 85.4 |
Redundancy | 6.8 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M PCTP buffer pH6, 25% (w/v) PEG 1500 |