6BWJ

Crystal structure of the TRPV2 ion channel in complex with RTx

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	90.054, 121.226, 185.557
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.997 - 3.100
R-factor	0.2488
Rwork	0.247
R-free	0.27190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5an8
RMSD bond angle	0.665
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	185.560	3.210
High resolution limit [Å]	3.100	3.100
Rpim	0.160	1.670
Number of reflections	37513
<I/σ(I)>	8.6
Completeness [%]	100.0
Redundancy	13.7
CC(1/2)	0.990	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	PEG 400, Calcium chloride