6BW5

Human GPT (DPAGT1) in complex with tunicamycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	212.272, 105.512, 149.389
Unit cell angles	90.00, 103.53, 90.00

Refinement procedure

Resolution	83.048 - 3.100
R-factor	0.2577
Rwork	0.256
R-free	0.28840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lev
RMSD bond length	0.003
RMSD bond angle	0.672
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.211
High resolution limit [Å]	3.100	3.100
Rpim	0.068
Number of reflections	35101	5815
<I/σ(I)>	13.65
Completeness [%]	97.5	96.29
Redundancy	79.9	69.1
CC(1/2)	0.963	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	PEG400, calcium chloride