6BFT

Structure of Bevacizumab Fab mutant in complex with VEGF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	83.279, 88.749, 106.676
Unit cell angles	90.00, 111.87, 90.00

Refinement procedure

Resolution	45.000 - 2.550
R-factor	0.1727
Rwork	0.170
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bj1
RMSD bond length	0.010
RMSD bond angle	1.082
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.640
High resolution limit [Å]	2.550	2.550
Rpim	0.056
Number of reflections	47223
<I/σ(I)>	11.6
Completeness [%]	99.8
Redundancy	5.2
CC(1/2)		0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	100 mM MES, 6.0, 225 mM ammonium sulfate, 13% PEG4000, 10% isopropanol