6BE6

ADAM10 Extracellular Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	131.420, 188.780, 86.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.476 - 2.800
R-factor	0.2387
Rwork	0.236
R-free	0.28450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkc
RMSD bond length	0.004
RMSD bond angle	0.506
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.476	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	52456	5184
<I/σ(I)>	4.8	1.35
Completeness [%]	97.4	97.99
Redundancy	3.1	3.1
CC(1/2)	0.959	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.26M Ammonium Sulfate 100mM HEPES pH 7.5 21.75 % PEG8000