6BDZ

ADAM10 Extracellular Domain Bound by the 11G2 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	79.230, 97.580, 262.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.790 - 3.100
R-factor	0.2669
Rwork	0.261
R-free	0.31730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	0.693
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.100	3.100
Number of reflections	35539	1850
<I/σ(I)>	4.05	0.87
Completeness [%]	99.8	98.76
Redundancy	10.6	10.5
CC(1/2)	0.989	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	2.23M Ammonium Sulfate 100mM Tris pH 8.5 2% PEG400