6BC8
Crystal structure of Rev7-R124A/Rev3-RBM2 (residues 1988-2014) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 77.15 |
Detector technology | PIXEL |
Collection date | 2016-03-04 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 63.691, 63.691, 116.274 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 116.270 - 1.680 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ABD (Rev7 molecule only) |
RMSD bond length | 0.019 |
RMSD bond angle | 1.840 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 116.270 |
High resolution limit [Å] | 1.680 |
Rmerge | 0.063 |
Rmeas | 0.067 |
Rpim | 0.022 |
Number of reflections | 31583 |
<I/σ(I)> | 40.4 |
Completeness [%] | 99.6 |
Redundancy | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292.15 | well solution containing 100 mM sodium acetate, 200 mM NaCl, 1.4 M ammonium sulfate was mixed at 1:1 ratio with protein at 45 mg/mL in 5 mM HEPES, 100 mM NaCl, 10 mM DTT, pH=7.4 |