6BC0
A Complex between PH Domain of p190RhoGEF and Activated RhoA Bound to a GTP Analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 73.160, 73.160, 266.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.610 - 2.198 |
R-factor | 0.226 |
Rwork | 0.223 |
R-free | 0.28070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.344 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.610 | 2.277 |
High resolution limit [Å] | 2.198 | 2.198 |
Number of reflections | 22370 | 2178 |
<I/σ(I)> | 24.3 | |
Completeness [%] | 98.9 | 100 |
Redundancy | 11.2 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-24% PEG 3350, 100mM TrisCl, 200mM NaCl |