6B92

Crystal Structure of the N-terminal domain of human METTL16 in complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-25
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0000
Spacegroup name	I 41 3 2
Unit cell lengths	190.351, 190.351, 190.351
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.870 - 2.100
R-factor	0.182
Rwork	0.181
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h00
RMSD bond length	0.014
RMSD bond angle	1.676
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.064	1.360
Rmeas	0.066	1.410
Number of reflections	34224	5267
<I/σ(I)>	28.7	2
Completeness [%]	99.3	95.9
Redundancy	17.3	14.2
CC(1/2)	1.000	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	1.3 M K2HPO4, 45 mM NaH2PO4; drop contained 4 uL of protein solution (27 mg/mL) and 2 uL of reservoir solution; 0.2 uL of 200 mM SAM solution (buffered in 50 mM Hepes pH7.5) was added to the drop with mature crystals. 25% ethylene glycol was used for cryo-protection